NMR

The NMR instrumentation currently includes three spectrometers:

(i) An Agilent spectrometer operating at 14.1 T with 4 channels, and a 3 mm HCN cold probe. The system has full automation via autotune and cooled autosampler;

(ii) A Bruker 16.45 T four-channel dual-receiver spectrometer equipped with two cryoprobes and cooled sample changer

(iii) Bruker three-channel dual receiver wide bore 9.4 T microimaging/spectroscopy system for small animal and tissue-based imaging and spectroscopy. Data stations running NMR analysis software are part of the Center.

Agilent 14.1 T Spectrometer

The 14.1 T Agilent DD2 spectrometer is dedicated to metabolic studies. The instrument includes autotune for the probes, ensuring optimal pulse calibrations, with a 96-place random access cooled autosampler that can accept any tube size or configuration. The sample can be maintained at 5 °C to maintain integrity when larger numbers of samples are to analyzed. The instrument is equipped with two probes:

1. A room temperature 5 mm OneProbe capable of any double resonance experiments with 1H or 19F observe

2. A 3 mm cold HCN 13C-enhanced probe which is the workhorse metabolomics probe for proton-detected 13C or 15N SIRM experiments using small volume samples (< 100 µL with 3 mm sample tube; <50 µL with a 1.7 mm sample tube).

  • Direct 13C observe on this probe is more sensitive than a broad band RT probe.
  • Triple resonance with 2H decoupling is standard on the cold probe.

Bruker 16.45 T Spectrometer

The Bruker Avance III spectrometer features 4 channels, two receivers and a cooled SampleCase sample changer and two autotune cryoprobes.

1. 5 mm inverse quad probe (HCNP)

2. 1.7 mm HCN 13C-enhanced probe for proton-detected 13C or 15N SIRM experiments using small volume samples (< 50 µL with 1.7 mm sample tube).

  • Direct 13C observe on this probe is more sensitive than a broad band RT probe 16.45 T Spectrometer

Bruker 9.4 T Wide Bore Spectrometer

The Bruker Avance III spectrometer features 3 channels, two receivers, microimaging with 120 G/cm x,y,z gradients, a proton volume imaging coil and surface coils tuned to 31P and 13C and a dual 13C/31P 1 cm surface coil for small animal spectroscopy. In addition there is a 10 mm XH probe for perfused cell or tissue spectrometry

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